Hydroxyproline Antibody #73812
Filter:
- IHC
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| SOURCE | Rabbit |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Description
Hydroxyproline Antibody provides a means of visualizing hydroxyproline in paraffin-embedded tissues. Using Hydroxyproline Antibody in the IHC assay, global collagen in tissue can be detected, while also providing contextual information that is lost in traditional hydroxyproline assays. Additionally, Hydroxyproline Antibody can be combined with other antibodies to enable detection of other markers of interest.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Hydroxyproline Antibody recognizes endogenous levels of proline only when the proline is hydroxylated. The antibody does not react with non-hydroxylated proline.
Species Reactivity:
All Species Expected
Source / Purification
Polyclonal antibodies are produced by immunizing animals with an antigen containing hydroxyproline. Antibodies are purified by affinity chromatography.
Background
Collagens are a large family of proteins and collectively they are the most abundant protein in mammals. They are trimeric molecules comprised of three alpha polypeptide chains that form a triple helix structure that is characteristic of all collagens (1). Collagen chains have important features that allow helix to form: every third amino acid in the sequence is a glycine, and the chains are co- and post-translationally modified. Prolines are hydroxylated by collagen prolyl 4-hydroxylases. These hydroxyproline modifications are critical for the stability of the triple helix to form fibrils (2).
While proline hydroxylation occurs on a small number of other proteins, the hydroxyproline content of tissue comes almost entirely from collagen, such that hydroxyproline quantitative biochemical assays are routinely utilized as a measurement of collagen (3).
While proline hydroxylation occurs on a small number of other proteins, the hydroxyproline content of tissue comes almost entirely from collagen, such that hydroxyproline quantitative biochemical assays are routinely utilized as a measurement of collagen (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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