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Render Timestamp: 2025-01-09T07:43:12.435Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-10-31 19:07:10.419
Product last modified at: 2025-01-01T09:01:13.247Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Human CD28 Activating (CD28.2) Mouse mAb (Low Endotoxin, Azide-free) #91920

Human CD28 Activating (CD28.2) Mouse mAb (Low Endotoxin, Azide-free): Image 1
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with cross-linked Human CD3ε Activating (OKT3) Mouse mAb (Low Endotoxin, Azide-free) #92511 and Human CD28 Activating (CD28.2) Mouse mAb (Low Endotoxin, Azide-free) (10 μg/ml each, 15 min; green), using Phospho-SLP-76 (Ser376) (E3G9U) XP® Rabbit mAb #76384 (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

To Purchase # 91920S

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG1 kappa
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products
  • Conjugates

Product Information

Product Description

Endotoxin Less than or equal to 0.01 EU/μg, as determined by the LaL assay.

Formulation

Supplied in 10 mM NaH2PO4, 150 mM NaCl, pH7.2, Low Endotoxin, Azide-free.

Storage

Stable for 12 months when stored at 4°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Human CD28 Activating (CD28.2) Mouse mAb (Low Endotoxin, Azide-free) recognizes endogenous levels of CD28 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced using cells transfected with human CD28 and purified via affinity chromatography.

Background

CD28 is a transmembrane glycoprotein expressed by T cells as well as some other hematopoietic cells (1, 2). T cell activation requires T cell receptor (TCR) recognition of antigen presented in the context of MHC molecules. CD28 acts as a T cell costimulatory receptor, and interaction of CD28 with its ligands CD80 or CD86 provides the second signal required for naïve T cell activation (3-5). Activation of naïve T cells in the absence of CD28 stimulation can result in a state of T cell anergy, or unresponsiveness (3). CD28 signals through cytoplasmic phospho-tyrosine motifs that bind several SH2 or SH3 domain-containing proteins involved in T cell activation (2). Recently, CD28 was demonstrated to be a preferred target of PD-1-mediated dephosphorylation. Consistently, CD28 expression was required for T cell proliferation following PD-1 blockade and CD28 stimulation was required for effective anti-PD-1 cancer immunotherapy in mice (6, 7). Several CD28 isoforms are produced by alternative splicing (8).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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