PhosphoPlus® HSP27 (Ser82) Antibody Duet #11900
Western blot analysis of extracts from HeLa cells (lane 1) or HSP27 knock-out cells (lane 2) using HSP27 (D6W5V) Rabbit mAb #95357 (upper), and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The absence of signal in the HSP27 knock-out HeLa cells confirms specificity of the antibody for HSP27.
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| Product Includes | Quantity | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|
| Phospho-HSP27 (Ser82) (D1H2F6) XP® Rabbit mAb #9709 | 100 µl | H M | 27 | Rabbit IgG |
| HSP27 (D6W5V) Rabbit mAb #95357 | 100 µl | H | 27 | Rabbit IgG |
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Kit Usage Information
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Product Description
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
Background
Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).
Pathways
Explore pathways related to this product.
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