HRS Antibody #14346
Inquiry Info. # 14346
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Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
HRS Antibody recognizes endogenous levels of total HRS protein.
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly670 of human HRS protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) is a ubiquitously expressed, multidomain-containing protein that is tyrosine phosphorylated upon activation of multiple receptor tyrosine kinases (1). HRS contains a proline-rich region, which may mediate interactions with SH3 domain-containing proteins (1). Research studies have also demonstrated that HRS possesses a phosphatidylinositol 3-phosphate-binding FYVE-type zinc finger domain and a coiled-coil domain that target it to membranes of the endosomal compartment (2-4). HRS also possesses a ubiquitin-interacting motif (UIM) that binds ubiquitinated membrane proteins and, in conjunction with Eps15 and STAM proteins of the ESCRT-0 complex, facilitates their sorting through the endosomal compartment for eventual degradation in the lysosome (5-8). Research studies demonstrate that phosphorylation and ubiquitination of HRS play a role in EGFR intracellular trafficking and degradation (9,10).
- Komada, M. and Kitamura, N. (1995) Mol Cell Biol 15, 6213-21.
- He, J. et al. (2009) Proteins 76, 852-60.
- Raiborg, C. et al. (2001) J Cell Sci 114, 2255-63.
- Komada, M. et al. (1997) J Biol Chem 272, 20538-44.
- Raiborg, C. et al. (2002) Nat Cell Biol 4, 394-8.
- Hirano, S. et al. (2006) Nat Struct Mol Biol 13, 272-7.
- Bache, K.G. et al. (2003) J Biol Chem 278, 12513-21.
- Urbé, S. et al. (2003) J Cell Sci 116, 4169-79.
- Stern, K.A. et al. (2007) Mol Cell Biol 27, 888-98.
- Tan, X. et al. (2015) EMBO J 34, 475-90.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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