HOXD9 Antibody #62883
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
HOXD9 Antibody recognizes endogenous levels of total HOXD9 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HOXD9 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Homeobox protein Hox-D9 (HOXD9) is a sequence-specific transcription factor that is part of a developmental regulatory program that provides cells with specific positional identities on the anterior-posterior axis. HOXD9 is developmentally expressed in structures of either mesodermal or neuro-ectodermal origin, such as developing limbs, gonads, and the central nervous system (1-6). HOXD9 plays a critical role in regulation of limb development, neuronal development, and development of mammary glands and gonads in many organisms (1-6). The HOXD9 gene promoter is found to be hypermethylated and silenced in multiple types of cancer, including breast cancer, melanoma brain metastases, and cholangiocarcinomas (7-9). In addition, HOXD9 expression is increased in other types of cancer, including human glioblastomas and astrocytomas, where expression appears to drive growth of the tumors (10).
- Raines, A.M. et al. (2013) Development 140, 2942-52.
- Wang, Z. et al. (2010) BMC Genomics 11, 619.
- Freitas, R. et al. (2007) PLoS One 2, e754.
- Tanaka, M. et al. (2005) Dev Biol 281, 227-39.
- de la Cruz, C.C. et al. (1999) Dev Biol 216, 595-610.
- Chen, F. and Capecchi, M.R. (1999) Proc Natl Acad Sci U S A 96, 541-6.
- Makiyama, K. et al. (2005) Oncol Rep 13, 673-9.
- Marzese, D.M. et al. (2014) Hum Mol Genet 23, 226-38.
- Sriraksa, R. et al. (2013) Cancer Prev Res (Phila) 6, 1348-55.
- Tabuse, M. et al. (2011) Mol Cancer 10, 60.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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