R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
HOIP/RNF31 (E6M5B) Rabbit mAb #99633
Filter:
- WB
- IP
Supporting Data
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 120 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
HOIP/RNF31 (E6M5B) Rabbit mAb recognizes endogenous levels of total HOIP/RNF31 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HOIP/RNF31 protein.
Background
HOIL-1-interacting protein (HOIP/RNF31), a RING-type E3 ubiquitin ligase, is the catalytic subunit of the Linear Ubiquitin Chain Assembly Complex (LUBAC) that is associated with TNF-R1 (1). Research studies have shown that the LUBAC consists of three subunits: HOIP, HOIL-1L, and Sharpin that facilitate canonical NF-kB activation in response to pro inflammatory cytokines through M1-linked linear ubiquitination of NEMO and RIP1 (2-6). As part of the LUBAC, HOIP has also been implicated in the negative regulation of interferon-mediated antiviral signaling through the suppression of RIG-I activation (7). The role of HOIP in LUBAC function and human disease is underscored by naturally occurring mutations in HOIP that impair LUBAC assembly and NF-kb activation. Patients that are homozygous for this mutation in HOIP have multi organ auto inflammation and immunodeficiency (8).
- Haas, T.L. et al. (2009) Mol Cell 36, 831-44.
- Smit, J.J. et al. (2012) EMBO J 31, 3833-44.
- Tokunaga, F. et al. (2009) Nat Cell Biol 11, 123-32.
- Tokunaga, F. et al. (2011) Nature 471, 633-6.
- Blackwell, K. et al. (2013) Mol Cell Biol 33, 1901-15.
- Ikeda, F. et al. (2011) Nature 471, 637-41.
- Inn, K.S. et al. (2011) Mol Cell 41, 354-65.
- Boisson, B. et al. (2015) J Exp Med 212, 939-51.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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