hnRNP Q/R Antibody #5351
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75-82 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
hnRNP Q/R Antibody detects endogenous levels of hnRNP Q and hnRNP R proteins.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human hnRNP Q. Antibodies were purified by protein A and peptide affinity chromatography.
Background
Heterogeneous nuclear ribonucleoprotein Q and R belong to a family of hnRNP proteins that are involved in RNA binding, RNA biosynthesis, and mRNA transport from the nucleus to the cytoplasm (1-3). These two proteins are encoded by different genes but have 83% homology. hnRNP Q has three alternative splice variants (hnRNP Q1-3) (1-3). Methylation of carboxy-terminal arginine residues is required for nuclear localization (4). hnRNP Q binds to AU-rich mRNA in conjunction with AUF1 and regulates mRNA decay (5). hnRNP Q isoforms play a crucial role in mediating nuclear function of survival of motor neuron (SMN) complex (6,7) and modulating RNA biosynthesis and hepatitis C virus replication (8). hnRNP R was identified recently and its function is still under investigation (9), however hnRNP R does not duplicate the biological function of hnRNP Q. Both hnRNP Q and R are present in cytoplasmic mRNP granules containing untranslated mRNAs (10) and both interact with SMN (6).
- Dreyfuss, G. et al. (1993) Annu Rev Biochem 62, 289-321.
- Burd, C.G. and Dreyfuss, G. (1994) Science 265, 615-21.
- Krecic, A.M. and Swanson, M.S. (1999) Curr Opin Cell Biol 11, 363-71.
- Passos, D.O. et al. (2006) Biochem Biophys Res Commun 346, 517-25.
- Moraes, K.C. et al. (2003) Biol Chem 384, 25-37.
- Mourelatos, Z. et al. (2001) EMBO J 20, 5443-52.
- Rossoll, W. et al. (2002) Hum Mol Genet 11, 93-105.
- Liu, H.M. et al. (2009) Virology 386, 249-56.
- Hassfeld, W. et al. (1998) Nucleic Acids Res 26, 439-45.
- Carty, S.M. and Greenleaf, A.L. (2002) Mol Cell Proteomics 1, 598-610.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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