HLTF Antibody #43345
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
HLTF Antibody recognizes endogenous levels of total HLTF protein.
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala998 of human HLTF protein. Antibodies are purified by peptide affinity chromatography.
Background
Helicase-like transcription factor (HLTF), also known as RUSH-1 or SMARCA3, belongs to the SNF2/SWI2 family of ATP-dependent remodelers (1-3). HLTF has been shown to interact with ubiquitin enzyme complexes to promote Lys-63-linked polyubiquitination of PCNA, maintaining genomic stability during DNA damage (4,5). HLTF has double-stranded DNA translocation activity, which can restart replication forks at DNA lesions, providing tumor suppressive functions (6). The HLTF gene is frequently hypermethylated in many different tumor types (7-9).
- Sheridan, P.L. et al. (1995) J Biol Chem 270, 4575-87.
- Ding, H. et al. (1996) DNA Cell Biol 15, 429-42.
- Hayward-Lester, A. et al. (1996) Mol Endocrinol 10, 1335-49.
- Unk, I. et al. (2008) Proc Natl Acad Sci U S A 105, 3768-73.
- Motegi, A. et al. (2008) Proc Natl Acad Sci U S A 105, 12411-6.
- Blastyák, A. et al. (2010) Mol Cell Biol 30, 684-93.
- Moinova, H.R. et al. (2002) Proc Natl Acad Sci U S A 99, 4562-7.
- Hibi, K. et al. (2003) Int J Cancer 104, 433-6.
- Leung, W.K. et al. (2003) Mol Carcinog 37, 91-7.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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