R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
HJURP (D3A8Z) Rabbit mAb #80508
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
HJURP (D3A8Z) Rabbit mAb recognizes endogenous levels of total HJURP protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly672 of human HJURP protein.
Background
CENP-A is an essential histone H3 variant that replaces canonical histone H3 in centromeric heterochromatin. The inherited localization of the centromere is specified by CENP-A (1). CENP-A deposition to the correct chromosomal location in early G1 phase is regulated by the Mis18 complex, which recruits the CENP-A assembly factor/chaperone protein HJURP (Holliday Junction Recognition Protein) (2-3).
Dimerization of HJURP is required for its activity (4), and phosphorylation by cyclin dependent kinases is required for temporal regulation of HJURP recruitment (5).
Overexpression of HJURP causes chromosome loss in yeast and mitotic defects in mammalian cells (6). Further, downregulation of HJURP expression has been associated with replicative senescence in human cells (7).
Research studies indicate that HJURP may have prognostic value in human breast cancer and high grade gliomas (8-10).
Dimerization of HJURP is required for its activity (4), and phosphorylation by cyclin dependent kinases is required for temporal regulation of HJURP recruitment (5).
Overexpression of HJURP causes chromosome loss in yeast and mitotic defects in mammalian cells (6). Further, downregulation of HJURP expression has been associated with replicative senescence in human cells (7).
Research studies indicate that HJURP may have prognostic value in human breast cancer and high grade gliomas (8-10).
- Ausió, J. (2006) Brief Funct Genomic Proteomic 5, 228-43.
- Stellfox, M.E. et al. (2013) Cell Mol Life Sci 70, 387-406.
- Wang, J. et al. (2014) J Biol Chem 289, 8326-36.
- Zasadzińska, E. et al. (2013) EMBO J 32, 2113-24.
- Müller, S. et al. (2014) Cell Rep 8, 190-203.
- Mishra, P.K. et al. (2011) PLoS Genet 7, e1002303.
- Heo, J.I. et al. (2013) J Gerontol A Biol Sci Med Sci 68, 914-25.
- Hu, Z. et al. (2010) Breast Cancer Res 12, R18.
- Montes de Oca, R. et al. (2015) Mol Oncol 9, 657-74.
- de Tayrac, M. et al. (2013) PLoS One 8, e73332.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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