R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Histone H2A.B (F2J5F) Rabbit mAb #69900
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 12 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Histone H2A.B (F2J5F) Rabbit mAb recognizes endogenous levels of total histone H2A.B protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human histone H2A.B protein.
Background
Histones are essential proteins that compact eukaryotic DNA into chromatin and are the basis for the epigenetic control of genomic functions. Among the four core histones, the H2A family has the greatest sequence divergence that results in the largest number of variants, such as H2A.X, H2A.Z, and H2A.B, which play various biological functions (1). H2A.B shares only 48% sequence identity with the canonical H2A and is often associated with active gene transcription (2). H2A.B falls into the “short” variant category as it lacks the H2A carboxy terminus, which may notably alter its interactions and dynamic properties within the nucleosome core particle. The H2A.B histone variant appeared in eutherian mammals and is testis-specific (3,4), but it has also been reported to be abnormally expressed in Hodgkin lymphoma cell lines (5,6). Recently, it was demonstrated that H2A.B enhances ribosome biogenesis by increasing transcription of ribosomal DNA and ribosomal protein genes (7).
- Henikoff, S. and Smith, M.M. (2015) Cold Spring Harb Perspect Biol 7, a019364.
- González-Romero, R. et al. (2008) Gene 413, 1-7.
- Molaro, A. et al. (2018) Genome Res 28, 460-473.
- Jiang, X. et al. (2020) Cells 9, 867. doi: 10.3390/cells9040867.
- Winkler, C. et al. (2012) Cancer Immunol Immunother 61, 1769-79.
- Sansoni, V. et al. (2014) Nucleic Acids Res 42, 6405-20.
- Jiang, X. et al. (2021) EMBO Rep 22, e52462.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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