HIP1R Antibody #91617
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 138 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
HIP1R Antibody recognizes endogenous levels of total Huntingtin-interacting protein 1-related (HIP1R) protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Huntingtin-interacting protein 1-related (HIP1R) protein. Antibodies are purified by peptide affinity chromatography.
Background
Huntingtin-interacting protein 1-related protein, or HIP1R, was identified on the basis of its structural homology with Huntingtin-interacting protein 1, or HIP1. Based on its domain structure, HIP1R is a putative endocytosis-related protein. Knockdown of HIP1R impairs the endocytosis of activated epidermal growth factor receptor (EGFR) and the consequent activation of the downstream ERK and Akt proteins (1). Additionally, HIP1R is a component of the clathrin-coated pits and vesicles, which in part links the endocytic machinery to the actin cytoskeleton. It binds to 3-phosphoinositides via ENTH domains to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis (2,3). HIP1R deficiency significantly reduces the expression of the ionotropic glutamate receptor GluA1, GluN2A, and GluN2B subunits, but not the GABAA receptor α1 subunit. Knockdown of HIP1R reduces the amplitude and frequency of the miniature excitatory postsynaptic current, but not of the miniature inhibitory postsynaptic current (4). HIP1R has been identified as a protein that can target histone deacetylase-3-mediated neurodegeneration, along with other proteins like NPTX1, NFL, TEX10, and TGFFG (5).
- Yang, Q. et al. (2018) Front Mol Neurosci 11, 447.
- Akamatsu, M. et al. (2020) Elife 9, e49840. doi: 10.7554/eLife.49840.
- Engqvist-Goldstein, A.E. et al. (2001) J Cell Biol 154, 1209-23.
- Peng, L. et al. (2017) Front Mol Neurosci 10, 186.
- Qu, Z. and D'Mello, S.R. (2018) Exp Biol Med (Maywood) 243, 627-638.
限制使用
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