R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
HIP1 (E9J9B) Rabbit mAb #90830
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 116 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
HIP1 (E9J9B) Rabbit mAb recognizes endogenous levels of total HIP1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser166 of human HIP1 protein.
Background
Huntingtin-interacting protein 1 (HIP1), a ubiquitously expressed cytosolic protein, was originally discovered by yeast two-hybrid screening for proteins that interact with the huntingtin protein. HIP1 binds the amino terminus of the huntingtin protein. This interaction is weakened by huntingtin protein mutations that cause expanded polyglutamine repeats, suggesting that HIP1 may play a role in Huntington’s disease (HD) pathogenesis. HIP1, along with its human ortholog, HIP1-related protein (HIP1R) and its yeast ortholog Sla2p share a conserved domain structure; an epsin amino-terminal homology (ENTH) domain, a leucine zipper motif, and a carboxy-terminal domain homologous to TALIN (1,2). The ENTH domain of HIP1 binds clathrin and the adaptor protein AP-2, while the TALIN domain has been shown to bind actin. These interactions suggest that HIP1 plays a role in clathrin-mediated endocytosis (3-6). HIP1 has also been shown to bind receptor tyrosine kinases (RTKs), including the epidermal growth factor receptor (EGFR). Elevated expression levels of HIP1 correlate with elevated levels of EGFR and have been linked to the development and progression of various cancers (7-10).
- Rao, D.S. et al. (2002) J Clin Invest 110, 351-60.
- Rao, D.S. et al. (2001) Mol Cell Biol 21, 7796-806.
- Metzler, M. et al. (2001) J Biol Chem 276, 39271-6.
- Mishra, S.K. et al. (2001) J Biol Chem 276, 46230-6.
- Waelter, S. et al. (2001) Hum Mol Genet 10, 1807-17.
- Senetar, M.A. et al. (2004) Biochemistry 43, 15418-28.
- Marghalani, S. et al. (2015) Int J Dermatol 54, 640-7.
- Ames, H.M. et al. (2013) Mol Cell Biol 33, 3580-93.
- Rao, D.S. et al. (2003) Cancer Cell 3, 471-82.
- Bradley, S.V. et al. (2007) Cancer Res 67, 3609-15.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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