R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
HIF-2α (E2N9W) Rabbit mAb #57921
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 120 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
HIF-2α (E2N9W) Rabbit mAb recognizes endogenous levels of total HIF-2α protein. This antibody does not cross-react with HIF-1α protein.
Species Reactivity:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse HIF-2α protein.
Background
Hypoxia-inducible factor (HIF) is essential for the cellular response to hypoxia (1,2). Under normoxia conditions, the α subunit of HIF is ubiquitinated by von Hippel-Lindau (VHL) protein and is degraded in the ubiquitin/proteasome pathway (1,2). Hypoxia inhibits degradation of the α subunit, which leads to its stabilization (1,2). HIF, in turn, regulates the transcription of a variety of genes that respond to hypoxia conditions (1,2). There are several isoforms of the HIF α subunit (2). Studies have found that HIF-1α and HIF-2α expression is increased in some human cancers (2). HIF-1α has both pro- and anti-proliferative activities, whereas HIF-2α does not possess anti-proliferative activity (2). Therefore, HIF-2α likely plays an important role in tumorigenesis (2,3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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