渲染靶标:SSR
Render Timestamp: 2025-01-25T05:21:26.336Z
Commit: 199712eb9daea12d88cc0e67894a8a09f475f8cb
XML generation date: 2024-09-20 06:15:37.050
Product last modified at: 2025-01-01T09:05:01.173Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Hexokinase I (C35C4) Rabbit mAb #2024

Filter:
  • WB
  • IP
  • IHC
  • IF
  • F
Western Blotting Image 1: Hexokinase I (C35C4) Rabbit mAb
Western blot analysis of extracts from various cell types using Hexokinase I (C35C4) Rabbit mAb.

To Purchase # 2024T

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 102
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:800
Immunofluorescence (Frozen) 1:800 - 1:1600
Immunofluorescence (Immunocytochemistry) 1:200
Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #75806.

Protocol

Specificity / Sensitivity

Hexokinase I (C35C4) Rabbit mAb detects endogenous levels of total hexokinase I protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human hexokinase I.

Background

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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