R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
HEXIM1 (D5Y5K) Rabbit mAb #12604
Filter:
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:1200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
HEXIM1 (D5Y5K) Rabbit mAb recognizes endogenous levels of total HEXIM1 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly63 of human HEXIM1 protein.
Background
Hexamethylene bis-acetamide-inducible protein 1 (HEXIM1) was originally identified in vascular smooth muscle cells as a protein that is upregulated upon treatment with the differentiating agent hexamethylene bisacetamide (1). HEXIM1 binds 7SK RNA, a highly abundant non-coding RNA, and together they act as a potent inhibitor of positive transcription elongation factor b (P-TEFb) (2,3). P-TEFb phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II and is an important regulator of transcription elongation (4-8). 7SK RNA-bound HEXIM1 interacts with the cyclin T1 subunit of P-TEFb, sequestering P-TEFb in an inactive form leading to transcription inhibition (2,3). The regulation of the relative ratio of inactive to active P-TEFb in the cell by HEXIM1/7SK RNA is thought to play a critical role in regulation of a wide range of cellular gene expression programs such as estrogen and glucocorticoid receptor regulated genes (9-12).
- Ouchida, R. et al. (2003) Genes Cells 8, 95-107.
- Michels, A.A. et al. (2004) EMBO J 23, 2608-19.
- Yik, J.H. et al. (2003) Mol Cell 12, 971-82.
- Buratowski, S. (2009) Mol Cell 36, 541-6.
- Lenasi, T. and Barboric, M. RNA Biol 7, 145-50.
- Pirngruber, J. et al. (2009) Cell Cycle 8, 3636-42.
- Wada, T. et al. (1998) EMBO J 17, 7395-403.
- Yamada, T. et al. (2006) Mol Cell 21, 227-37.
- Peterlin, B.M. et al. (2012) Wiley Interdiscip Rev RNA 3, 92-103.
- Ketchart, W. et al. (2011) Oncogene 30, 3563-9.
- Ogba, N. et al. (2008) Cancer Res 68, 7015-24.
- Shimizu, N. et al. (2005) Proc Natl Acad Sci U S A 102, 8555-60.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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