HAT1 Antibody #15348
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 49 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
HAT1 Antibody recognizes endogenous levels of total HAT1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val412 of human HAT1 protein. Antibodies are purified by peptide affinity chromatography.
Background
Histone acetyltransferase 1 (HAT1) is a member of the GCN5 family of acetyltransferases that can acetylate Lys5 and Lys12 on newly generated histone H4, a process that is essential for mammalian development (1-5). HAT1 binds to the H4 gene promoter, where it facilitates the production and acetylation of new histones. This process is strongly dependent on glucose availability, suggesting HAT1 plays a role in nutrient sensing (6). HAT1 has also been shown to contribute to recovery from replication-based DNA damage by incorporating H4Lys5/Lys12 acetylated histones at the sites of double-stranded breaks (7-9). HAT1 can acetylate non-histone proteins as well, and has been shown to play a key role in NF-kB signaling by acetylating PLZF, which in turn forms a complex with p50 to limit the production of cytokines (10). HAT1 overexpression has also been implicated in many cancer types (11-13).
- Kleff, S. et al. (1995) J Biol Chem 270, 24674-7.
- Verreault, A. et al. (1998) Curr Biol 8, 96-108.
- Ruiz-García, A.B. et al. (1998) J Biol Chem 273, 12599-605.
- Dutnall, R.N. et al. (1998) Cell 94, 427-38.
- Nagarajan, P. et al. (2013) PLoS Genet 9, e1003518.
- Gruber, J.J. et al. (2019) Mol Cell 75, 711-724.e5.
- Barman, H.K. et al. (2006) Biochem Biophys Res Commun 345, 1547-57.
- Benson, L.J. et al. (2007) J Biol Chem 282, 836-42.
- Yang, X. et al. (2013) J Biol Chem 288, 18271-82.
- Sadler, A.J. et al. (2015) Nat Commun 6, 6795.
- Min, S.K. et al. (2012) Korean J Pathol 46, 142-50.
- Miao, B.P. et al. (2018) Arch Biochem Biophys 646, 72-79.
- Fan, P. et al. (2019) J Exp Clin Cancer Res 38, 47.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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