R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Haspin (E7V4I) Rabbit mAb #52601
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 95 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Haspin (E7V4I) Rabbit mAb recognizes endogenous levels of total Haspin protein. This antibody cross-reacts with a band of unknown origin at approximately 62 kDa in some cell lines.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp70 of human Haspin protein.
Background
Haspin (Haploid germ cell-specific nuclear protein kinase, GSG2) is an atypical serine/threonine kinase missing the canonical DFG motif that is present in conventional protein kinases (1). Haspin phosphorylates histone H3 at Thr3 (2). This phosphorylation is important for cell cycle progression, positioning Aurora B kinase at centromeres to regulate centromeric sister chromatid cohesion during prophase and prometaphase, and subsequently ensuring proper chromosome alignment during metaphase and normal chromosome segregation during anaphase (3,4). Haspin has been shown to be overexpressed in multiple human tumor types, with roles in tumor progression and proliferation (5). Inhibition of Haspin kinase is a potential therapeutic approach for cancer treatment (6,7). In addition to cell cycle control, the phosphorylation of histone H3 at Thr3 by Haspin regulates primary cilia length and intracellular homeostasis (8).
- Eswaran, J. et al. (2009) Proc Natl Acad Sci U S A 106, 20198-203.
- Dai, J. et al. (2005) Genes Dev 19, 472-88.
- Wang, F. et al. (2010) Science 330, 231-5.
- Kelly, A.E. et al. (2010) Science 330, 235-9.
- Quadri, R. et al. (2022) Cell Signal 93, 110303.
- Wang, P. et al. (2020) J Cell Physiol 235, 4508-4519.
- Melms, J.C. et al. (2020) Cancer Res 80, 798-810.
- Quadri, R. et al. (2021) Int J Mol Sci 22, 7753.
限制使用
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专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
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