Render Target: SSR
Render Timestamp: 2024-11-21T10:54:48.398Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:28:50.298
Product last modified at: 2024-11-14T17:00:09.340Z
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PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c

GST-Tag (91G1) Rabbit mAb #2625

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GST-Tag (91G1) Rabbit mAb detects transfected GST fusion proteins.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a GST fusion protein.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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