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GSK-3β-Tag Antibody

GSK-3β-Tag Antibody #9325

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Supporting Data

REACTIVITY	All
SENSITIVITY	Transfected Only
MW (kDa)
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

GSK-3β-Tag Antibody is optimized to detect transfected levels of GSK-3β-tagged proteins and weakly detects endogenous GSK-3β (46 kDa).

Species Reactivity:

All Species Expected

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic GSK-3β-Tag peptide. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

Short phosphorylatable peptide tags, such as the GSK-3β-tag, can be used to determine translocation of effector proteins in type III secretion systems (1).

Hohlfeld, S. et al. (2006) Mol. Microbiol. 59, 1624-1637.

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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