GRK2 Antibody #3982
Inquiry Info. # 3982
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Supporting Data
| REACTIVITY | H M R Hm Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Hm-Hamster
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:25 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
GRK2 Antibody detects endogenous levels of total GRK2. This antibody may also detect GRK3, but does not cross-react with other GRKs.
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human GRK2. Antibodies are purified by protein A and peptide affinity chromatography.
Background
G protein-coupled receptor kinase 2 (GRK2), also known as beta-adrenergic receptor kinase 1 (beta-ARK1), is a member of the GRK family, which phosphorylates the activated form of G protein-coupled receptors (GPCRs) and initiates the desensitization process of GPCR (1). GRK2 kinase activity and cellular localization are tightly regulated by interactions with activated receptors, G-beta and G-gamma subunits, adaptor proteins, phospholipids, caveolin, and calmodulin, as well as by phosphorylation (1). Protein kinase C (PKC) phosphorylation enhances GRK2 activity by promoting its membrane localization and by abolishing the inhibitory association of calmodulin (2,3). Protein kinase A (PKA) phosphorylates GRK2 at Ser685, facilitating the association of GRK2 with a beta-adrenergic receptor (4). Erk inhibits GRK2 activity via phosphorylation at Ser670 (5). Src phosphorylates GRK2 at multiple tyrosine residues (Tyr13, 86, and 92), which activates GRK2 activity and promotes GRK2 degradation (6,7).
- Pitcher, J. A. et al. (1998) Annu. Rev. Biochem. 67, 653-692.
- Winstel, R. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 2105-2109.
- Krasel, C. et al. (2001) J. Biol. Chem. 276, 1911-1915.
- Cong, M. et al. (2001) J. Biol. Chem. 276, 15192-15199.
- Pitcher, J. A. et al. (1999) J. Biol. Chem. 274, 34531-34534.
- Sarnago, S. et al. (1999) J. Biol. Chem. 274, 34411-34416.
- Penela, P. et al. (2001) EMBO J. 20, 5129-5138.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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