Render Target: SSR
Render Timestamp: 2025-03-16T12:05:59.422Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2024-08-01 15:24:50.784
Product last modified at: 2025-02-19T23:30:09.734Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

GRB2 Antibody #3972

Filter:
  • WB
Western Blotting Image 1: GRB2 Antibody
Western blot analysis of extracts from HeLa, A431, HEK293, Ramos, NIH/3T3, C6, COS, CHO and BAEC cells, using GRB2 Antibody.

To Purchase # 3972

Cat. #

Size

Price

Inventory

3972S
100 µl
¥4,550

库存紧张

Supporting Data

REACTIVITY H M R Hm Mk B
SENSITIVITY Endogenous
MW (kDa) 25
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Hm-Hamster 
  • Mk-Monkey 
  • B-Bovine 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

GRB2 Antibody detects endogenous levels of total GRB2 protein.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey, Bovine

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human GRB2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Growth factor receptor-binding protein 2 (GRB2) is an adaptor protein that is involved in RTK signal transduction. The SH2 domain of GRB2 binds to tyrosine phosphorylated proteins such as EGFR, IRS-1, Shc and Gab1 (1). The SH3 domain of GRB2 associates with Sos, which stimulates the GTP binding activity of Ras, leading to the activation of the MAP kinase and other signaling pathways. Phosphorylation of Tyr209 of GRB2 by Bcr-Abl and EGFR abolishes its association with Sos and negatively regulates downstream signaling (2).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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