R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
GPAT3 (E5R1H) Rabbit mAb #71236
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
GPAT3 (E5R1H) Rabbit mAb recognizes endogenous levels of total GPAT3 protein. Background bands of unknown identity are detected at 20, 70, and 170 kDa.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His289 of human GPAT3 protein.
Background
Glycerol-3-phosphate acyltransferases (GPATs) catalyze the first step in the de novo synthesis of triacylglycerol (TAG) and phospholipids by converting glycerol-3-phosphate to lysophosphatidic acid (LPA) (1). The GPAT family consists of four family members: two mitochondrial proteins (GPAT1 and GPAT2) and two microsomal proteins (GPAT3 and GPAT4). Each can differ in tissue expression, regulation, and physiological and pathophysiological roles (2). The microsomal proteins GPAT3 and GPAT4 were originally designated as AGPAT8 and AGPAT6, respectively. 1-acyl-glycerol-3-phosphate acyltransferase (AGPAT) catalyzes the subsequent reaction converting LPA to phosphatidic acid. The genes were renamed to GPAT3 and GPAT4 upon elucidation of GPAT activity (3,4). Both GPAT3 and GPAT4 are expressed in adipose tissue, but GPAT3 is highly induced during adipocyte differentiation (4). Brown adipose tissue (BAT) preferentially expresses GPAT4 (5). GPAT3 and GPAT4 may also be phosphorylated in response to insulin treatment to increase GPAT activity (6). Accumulation of saturated LPA produced by GPAT4 at the ER-mitochondrial contact site can inhibit autophagy through the abnormal formation of omegasomes, which are precursors to the autophagosome (7). Glycerolipid synthesis by GPAT3 and GPAT4 can also regulate activation of macrophages (8).
- Yamashita, A. et al. (2014) Biology (Basel) 3, 801-30.
- Karasawa, K. et al. (2019) Int J Mol Sci 20, 964. doi: 10.3390/ijms20040964.
- Chen, Y.Q. et al. (2008) J Biol Chem 283, 10048-57.
- Cao, J. et al. (2006) Proc Natl Acad Sci U S A 103, 19695-700.
- Cooper, D.E. et al. (2015) J Biol Chem 290, 15112-20.
- Shan, D. et al. (2010) J Lipid Res 51, 1971-81.
- Shiozaki, Y. et al. (2020) iScience 23, 101105.
- Quiroga, I.Y. et al. (2019) Biochem J 476, 85-99.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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