R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Glut2 (F6X2X) Rabbit mAb #90520
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50-80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Glut2 (F6X2X) Rabbit mAb recognizes endogenous levels of total Glut2 protein. This antibody does not cross-react with Glut1, Glut3, Glut4, or Glut5 protein. This antibody detects a 35 kDa and a 160 kDa protein of unknown identity in some cell lines.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Glut2 protein.
Background
Glucose transporter 2 (Glut2), also known as SLC2A2, is a facilitated hexose transporter. It transports glucose, fructose, and galactose (1-3). Glut2 is expressed in various tissues, such as liver, small intestine, kidney, pancreatic β cells, and central nervous system (1,4). In addition, Glut2 is a marker of mature pancreatic β cells and is required for glucose-stimulated insulin secretion there (1,5). Furthermore, a mutation on human Glut2 protein expressed in Xenopus oocytes where Val197 is changed to isoleucine was shown to abolish its transport activity (6).
- Thorens, B. (2015) Diabetologia 58, 221-32.
- Manolescu, A. et al. (2005) J Biol Chem 280, 42978-83.
- Colville, C.A. et al. (1993) Biochem J 290 ( Pt 3), 701-6.
- Fukumoto, H. et al. (1988) Proc Natl Acad Sci USA 85, 5434-8.
- Deng, Z. et al. (2022) Mol Cell Biol 42, e0054121.
- Mueckler, M. et al. (1994) J Biol Chem 269, 17765-7.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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