R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
GLI3 (E6E2K) Rabbit mAb #71107
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 200 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
GLI3 (E6E2K) Rabbit mAb recognizes endogenous levels of total GLI3 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human GLI3 protein.
Background
GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Krüppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2, and GLI3 (3). These GLI proteins are similar to the Drosophila homolog Cubitus interruptus (Ci) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development, and research studies have shown that this pathway is aberrantly activated in many types of cancers (4,5).
Initially characterized as a negative regulator of Sonic hedgehog (Shh) signaling (6), follow-up studies described GLI3 as both a transcriptional activator and repressor, similar to GLI2 (7). GLI3 is upregulated in multiple cancers (8,9) but has also been shown to play an anti-cancerous role in acute myeloid leukemia (AML) and medulloblastoma (10,11).
Initially characterized as a negative regulator of Sonic hedgehog (Shh) signaling (6), follow-up studies described GLI3 as both a transcriptional activator and repressor, similar to GLI2 (7). GLI3 is upregulated in multiple cancers (8,9) but has also been shown to play an anti-cancerous role in acute myeloid leukemia (AML) and medulloblastoma (10,11).
- Kinzler, K.W. et al. (1987) Science 236, 70-3.
- Ruppert, J.M. et al. (1991) Mol Cell Biol 11, 1724-8.
- Kinzler, K.W. et al. (1988) Nature 332, 371-4.
- Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87.
- McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.
- Marigo, V. et al. (1996) Dev Biol 180, 273-83.
- Sasaki, H. et al. (1999) Development 126, 3915-24.
- Li, J. et al. (2018) Biochem Biophys Res Commun 503, 2101-2107.
- Ma, Y. et al. (2018) J Cell Biochem 119, 5324-5331.
- Miyahara, H. et al. (2014) Neuropathology 34, 1-10.
- Chaudhry, P. et al. (2017) Blood 129, 3465-3475.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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