R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
GLI2 (E5V8N) Rabbit mAb #18773
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 220 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
GLI2 (E5V8N) Rabbit mAb recognizes endogenous levels of total GLI2 protein.
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1188 of human GLI2 protein.
Background
GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Krüppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2, and GLI3 (3). These GLI proteins are similar to the Drosophila homolog Cubitus interruptus (Ci) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development, and research studies have shown that this pathway is aberrantly activated in many types of cancers (4,5).
GLI2 contains both transcription repression and activation domains (6), and several isoforms of GLI2 have been reported that may have different activities (7-9). Overexpression of GLI2 in skin causes basal cell carcinoma in mice (10), while loss-of-function of GLI2 is associated with pituitary anomalies (11).
GLI2 contains both transcription repression and activation domains (6), and several isoforms of GLI2 have been reported that may have different activities (7-9). Overexpression of GLI2 in skin causes basal cell carcinoma in mice (10), while loss-of-function of GLI2 is associated with pituitary anomalies (11).
- Kinzler, K.W. et al. (1987) Science 236, 70-3.
- Ruppert, J.M. et al. (1991) Mol Cell Biol 11, 1724-8.
- Kinzler, K.W. et al. (1988) Nature 332, 371-4.
- Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87.
- McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.
- Sasaki, H. et al. (1999) Development 126, 3915-24.
- Tanimura, A. et al. (1998) J Virol 72, 3958-64.
- Tojo, M. et al. (2003) Br J Dermatol 148, 892-7.
- Speek, M. et al. (2006) BMC Mol Biol 7, 13.
- Grachtchouk, M. et al. (2000) Nat Genet 24, 216-7.
- Roessler, E. et al. (2003) Proc Natl Acad Sci U S A 100, 13424-9.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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