GLI1 Antibody #2553
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 160 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
GLI1 Antibody detects endogenous levels of total GLI1 protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly420 of human GLI1. Antibodies are purified by protein A and peptide affinity chromatography
Background
GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Krüppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2, and GLI3 (3). These GLI proteins are similar to the Drosophila homolog Cubitus interruptus (Ci) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development, and research studies have shown that this pathway is aberrantly activated in many types of cancers (4,5).
GLI1 itself is a transcriptional target of the Hedgehog signaling pathway (6-8) and is used as a marker of Hedgehog signaling activation in cancer research (9,10).
GLI1 itself is a transcriptional target of the Hedgehog signaling pathway (6-8) and is used as a marker of Hedgehog signaling activation in cancer research (9,10).
- Kinzler, K.W. et al. (1987) Science 236, 70-3.
- Ruppert, J.M. et al. (1991) Mol Cell Biol 11, 1724-8.
- Kinzler, K.W. et al. (1988) Nature 332, 371-4.
- Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87.
- McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.
- Lee, J. et al. (1997) Development 124, 2537-2552.
- Dahmane, N. et al. (1997) Nature 389, 876-881.
- Dai, P. et al. (1999) J. Biol. Chem. 274, 8143-8152.
- Watkins, D.N. et al. (2003) Nature 422, 313-317.
- Karhadkar, S.S. et al. (2004) Nature 431, 707-712.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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