R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
GIRK2 (D6C2T) Rabbit mAb #25797
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 46 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
GIRK2 (D6C2T) Rabbit mAb recognizes endogenous levels of total GIRK2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg46 of human GIRK2 protein.
Background
GIRK2 is a member of G protein-coupled inwardly rectifying potassium channel family proteins (GIRKs). GIRK family proteins allow potassium to flow into the cell and therefore control cellular excitability in the central nervous system, heart, and pancreas (1-4). Activation of most GIRK channels requires heterologous subunit assembly and the presence of ATP (5-7). GIRK2 is abundantly expressed in the brain, where it is involved in pain perception. It is also required for peripheral opioid-mediated analgesia (8). Additionally GIRK2 localizes to pancreatic β cells and regulates insulin secretion (9,10). Mutations in the KCNJ6 gene encoding GIRK2 are associated with Keppen-Lubinsky Syndrome, a rare disease characterized by severe developmental delay, facial dysmorphism, and intellectual disability (11).
- Lesage, F. et al. (1995) J Biol Chem 270, 28660-7.
- Kubo, Y. et al. (1993) Nature 364, 802-6.
- Krapivinsky, G. et al. (1995) Nature 374, 135-41.
- Isomoto, S. et al. (1997) Jpn J Physiol 47, 11-39.
- Duprat, F. et al. (1995) Biochem Biophys Res Commun 212, 657-63.
- Corey, S. and Clapham, D.E. (1998) J Biol Chem 273, 27499-504.
- Han, J. et al. (2003) J Physiol 550, 693-706.
- Nockemann, D. et al. (2013) EMBO Mol Med 5, 1263-77.
- Ferrer, J. et al. (1995) J Biol Chem 270, 26086-91.
- Sakura, H. et al. (1995) FEBS Lett 367, 193-7.
- Masotti, A. et al. (2015) Am J Hum Genet 96, 295-300.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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