R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
GDNF (E5Y9N) Rabbit mAb #96166
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Recombinant and Transfected |
| MW (kDa) | Precursor 28 - Mature 13-16 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
GDNF (E5Y9N) Rabbit mAb recognizes human and mouse recombinant GDNF, and exogenously expressed GDNF protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu200 of human GDNF protein.
Background
Glial cell-derived neurotrophic factor (GDNF) plays an important role in the development and maintenance of the central and peripheral nervous system, renal morphogenesis, and spermatogenesis (1). This glycosylated, disulfide-bonded homodimer is a member of the TGF-β superfamily and plays an important role in neuronal survival (2). GDNF and the related GDNF family of ligands (GFLs) neurturin, persephin, and artemin bind to the GDNF family receptor α (GFRα) proteins that signal through the Ret receptor tyrosine kinase (3,4). The effect that GDNF has on degenerating dopamine neurons makes it an important growth factor when studying Parkinson’s disease (PD) (5). GDNF has also been identified as a therapeutic target for amyotrophic lateral sclerosis (ALS), is a suggested biomarker for Alzheimer’s Disease (AD), and has been associated with cancer progression (6-9).
- Fielder, G.C. et al. (2018) Neoplasia 20, 99-117.
- Choi-Lundberg, D.L. et al. (1997) Science 275, 838-41.
- Sariola, H. and Saarma, M. (2003) J Cell Sci 116, 3855-62.
- Tallini, G. and Asa, S.L. (2001) Adv Anat Pathol 8, 345-54.
- Konishi, Y. et al. (2014) J Neurosci 34, 13127-38.
- Cintrón-Colón, A.F. et al. (2020) Cell Tissue Res 382, 47-56.
- Sharif, M. et al. (2021) Neurol Sci 42, 2865-2872.
- Choi, Y.D. et al. (2020) Int J Mol Sci 21, 3586. doi: 10.3390/ijms21103586.
- Yu, Z. et al. (2022) Int J Oncol 61, 109. doi: 10.3892/ijo.2022.5399.
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