GCNF Antibody #5417
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
GCNF Antibody detects endogenous levels of total GCNF protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic polypeptide corresponding to residues surrounding Ala349 of human GCNF. Antibodies are purified by Protein A and peptide affinity chromatography.
Background
GCNF (Germ Cell Nuclear Factor), also known as NR6A1 (Nuclear Receptor Subfamily 6 Group A member), is an orphan member of the nuclear receptor gene superfamily (1). It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in gametogenesis, neurogenesis, and normal embryonic development during gastrulation (1,2). Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death (3). GCNF has been shown to be a repressor of OCT-4 and of the protamine genes (4,5) and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis (2,6).
- Lan, Z.J. et al. (2009) Biol Reprod 80, 905-12.
- Lei, W. et al. (1997) J Mol Endocrinol 18, 167-76.
- Chung, A.C. et al. (2001) Mol Cell Biol 21, 663-77.
- Akamatsu, W. et al. (2009) J Neurosci 29, 2113-24.
- Hummelke, G.C. and Cooney, A.J. (2004) Mol Reprod Dev 68, 394-407.
- Lan, Z.J. et al. (2002) J Biol Chem 277, 50660-7.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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