GATAD2A Antibody #19388
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70, 73 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
GATAD2A Antibody recognizes endogenous levels of total GATAD2A protein. This antibody does not cross-react with GATAD2B protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro56 of human GATAD2A protein. Antibodies are purified by peptide affinity chromatography.
Background
GATAD2A and GATAD2B, also known as p66 alpha and p66 beta, are related members of the nucleosome remodeling and deacetylase (NuRD) complex (1). GATAD2A interacts with MBD2 to recruit the deacetylase core of the NuRD complex to repress target genes, a process that is enhanced by the sumoylation of GATAD2A at Lys30 and Lys487 (2-5). In addition to being sumoylated, GATAD2A has also been shown to be phosphorylated by AMPK in the memory formation process (6). Depletion of GATAD2A in order to reduce NuRD-dependent repression of key pluripotency genes is being explored in the context of induced pluripotent stem cell (iPSC) generation (7,8).
- Brackertz, M. et al. (2002) J Biol Chem 277, 40958-66.
- Brackertz, M. et al. (2006) Nucleic Acids Res 34, 397-406.
- Gnanapragasam, M.N. et al. (2011) Proc Natl Acad Sci U S A 108, 7487-92.
- Desai, M.A. et al. (2015) Nucleic Acids Res 43, 3100-13.
- Gong, Z. et al. (2006) Mol Cell Biol 26, 4519-28.
- Ding, X. et al. (2017) Nat Neurosci 20, 690-9.
- Mor, N. et al. (2018) Cell Stem Cell 23, 412-425.e10.
- Zviran, A. et al. (2019) Cell Stem Cell 24, 328-341.e9.
限制使用
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