Render Target: SSR
Render Timestamp: 2025-04-01T20:55:53.991Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:16:26.122
Product last modified at: 2025-03-19T12:30:19.955Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Gasdermin D (E5O4N) Rabbit mAb #69469

Filter:
  • WB
  • IP
  • IHC
  • IF
Western Blotting Image 1: Gasdermin D (E5O4N) Rabbit mAb
Western blot analysis of extracts from control PC-3 cells (lane 1) or CRISPR/Cas9 Gasdermin D knockout (KO) PC-3 cells (lane 2) using Gasdermin D (E5O4N) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 69469

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 53, 30
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:50
Immunofluorescence (Immunocytochemistry) 1:800 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #96462.

Protocol

Specificity / Sensitivity

Gasdermin D (E5O4N) Rabbit mAb recognizes endogenous levels of total Gasdermin D protein. This antibody will recognize the 30 kDa amino-terminal fragment produced during pyroptosis.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to a fragment within the the amino-terminal pore-forming region of human Gasdermin D protein.

Background

Gasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSDMC, has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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