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XML generation date: 2024-08-01 15:30:43.598
Product last modified at: 2025-02-06T13:15:26.982Z
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1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. GABARAPL1 (D5R9Y) XP<sup>®</sup> Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GABARAPL1 (D5R9Y) XP® Rabbit mAb #26632

Filter:
  • WB
  • IF
  • F
Western Blotting Image 1: GABARAPL1 (D5R9Y) XP® Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human GABARAP protein (hGABARAP-Myc/DDK; +), human GABARAPL1 protein (hGABARAPL1-Myc/DDK; +), or human GABARAPL2 protein (hGABARAPL2-Myc/DDK; +) using GABARAPL1 (D5R9Y) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
1/4

To Purchase # 26632

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 14, 16
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:200
Flow Cytometry (Fixed/Permeabilized) 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #37737.

Protocol

Specificity / Sensitivity

GABARAPL1 (D5R9Y) XP® Rabbit mAb recognizes endogenous levels of total GABARAPL1 protein. This antibody does not cross react with other GABARAP family members.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GABARAPL1 protein.

Background

GABAA receptor associated protein (GABARAP) is an Atg8 family protein with a key role in autophagy, which was originally discovered as a protein associated with the GABAA receptor regulating receptor trafficking to the plasma membrane (1). Proteins in this family, including microtubule-associated protein light chain 3 (LC3) and GATE-16 (GABARAPL2), become incorporated into the autophagosomal membranes following autophagic stimuli such as starvation (2). Like the other family members, GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine (3,4). This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagosome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members (5,6) followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3 (7,8). GABARAPL1/GEC1, a protein that is highly related to GABARAP, was identified as an estrogen inducible gene, and is also associated with autophagosomes (9-11).
Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1) appears to be more highly expressed in the CNS as compared to other family members (12-14). Expression of GABARAPL1 is associated with prognosis of some cancers, including hepatocellular and breast cancer (15,16). Inhibition of GABARAPL1 expression in breast cancer cells attenuates autophagic flux, results in metabolic changes, and leads to cancer promoting activities (17).
  1. Wang, H. et al. (1999) Nature 397, 69-72.
  2. Shpilka, T. et al. (2011) Genome Biol 12, 226.
  3. Kabeya, Y. et al. (2004) J Cell Sci 117, 2805-12.
  4. Sou, Y.S. et al. (2006) J Biol Chem 281, 3017-24.
  5. Tanida, I. et al. (2004) J Biol Chem 279, 36268-76.
  6. Hemelaar, J. et al. (2003) J Biol Chem 278, 51841-50.
  7. Tanida, I. et al. (2001) J Biol Chem 276, 1701-6.
  8. Tanida, I. et al. (2002) J Biol Chem 277, 13739-44.
  9. Chakrama, F.Z. et al. (2010) Autophagy 6, 495-505.
  10. Pellerin, I. et al. (1993) Mol Cell Endocrinol 90, R17-21.
  11. Vernier-Magnin, S. et al. (2001) Biochem Biophys Res Commun 284, 118-25.
  12. Nemos, C. et al. (2003) Brain Res Mol Brain Res 119, 216-9.
  13. Wang, Y. et al. (2006) Neuroscience 140, 1265-76.
  14. Le Grand, J.N. et al. (2013) PLoS One 8, e63133.
  15. Liu, C. et al. (2014) Oncol Rep 31, 2043-8.
  16. Berthier, A. et al. (2010) Br J Cancer 102, 1024-31.
  17. Boyer-Guittaut, M. et al. (2014) Autophagy 10, 986-1003.

Database Links

UniProt ID: Q9H0R8


Entrez-Gene Id: 23710


限制使用

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