Render Target: SSR
Render Timestamp:
4/3/2025, 12:24:02 PM EDT
4/3/2025, 4:24:02 PM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:09:13.607
Product last modified at: 2025-03-06T12:30:48.343Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

GABA(B)R1 Antibody #3835

Filter:
  • WB
  • IP
Western Blotting Image 1: GABA(B)R1 Antibody
Western blot analysis of extracts from HeLa cells, co-transfected with constructs overexpressing GABA(B)R1 and GABA(B)R2, and mouse and rat brain using GABA(B)R1 Antibody.

To Purchase # 3835

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 130, 95
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

GABA(B)R1 Antibody detects endogenous levels of total GABA(B)R1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human GABA(B)R1. Antibodies are purified by peptide affinity chromatography.

Background

GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).
For Research Use Only. Not For Use In Diagnostic Procedures.
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