R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
G4S Linker (E7O2V) Human Chimeric mAb #57780
Filter:
- F
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Human IgG1 |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Description
This Cell Signaling Technology® antibody is tested in-house for indirect flow cytometric analysis in human cells and is expected to react with cell surface-expressed chimeric antigen receptors (CARs) of varying specificity, which contain a poly-Glycine-Serine (G4S) linker within the single-chain variable fragment (scFv) of the extracellular domain. Use of a fluorochrome-conjugated anti-human secondary antibody is recommended for detection in live cell flow cytometry assays.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Live) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
G4S Linker (E7O2V) Human Chimeric mAb recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing three Gly4Ser repeats. The VH domain and VL domain of G4S Linker (E7O2V) Rabbit mAb are formatted into an scFv that is joined to the hinge and Fc domain of human IgG1, resulting in a single-chain chimeric monoclonal antibody.
Background
The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S)3 or 20-mer (G4S)4 within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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