渲染靶标:SSR
Render Timestamp: 2025-03-31T09:14:48.513Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:06:36.055
Product last modified at: 2025-03-08T08:15:30.336Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

FoxP3 (D6O8C) Rabbit mAb #12632

Filter:
  • WB
  • F
Western Blotting Image 1: FoxP3 (D6O8C) Rabbit mAb
Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with human FoxP3 (hFoxP3; +), and human thymus using FoxP3 (D8O6C) Rabbit mAb.

To Purchase # 12632

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 45
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Flow Cytometry (Fixed/Permeabilized) 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #19713.

Protocol

Specificity / Sensitivity

FoxP3 (D6O8C) Rabbit mAb recognizes endogenous levels of total FoxP3 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro293 of human FoxP3 protein.

Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response, and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5).

FoxP3 is crucial for the development of T cells with regulatory properties (Treg) (6). Mutations in FoxP3 are associated with immune dysregulation, polyendocrinopathy, enteropathy, and X-linked syndrome (IPEX) (7), while overexpression in mice causes severe immunodeficiency (8). Research studies have shown that FoxP3 functions as a tumor suppressor in several types of cancer (9-11).
For Research Use Only. Not For Use In Diagnostic Procedures.
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