R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
FoxP3 (D25D4) Rabbit mAb #5298
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
FoxP3 (D25D4) Rabbit mAb detects endogenous levels of total FoxP3 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp295 of human FoxP3 protein.
Background
Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response, and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5).
FoxP3 is crucial for the development of T cells with regulatory properties (Treg) (6). Mutations in FoxP3 are associated with immune dysregulation, polyendocrinopathy, enteropathy, and X-linked syndrome (IPEX) (7), while overexpression in mice causes severe immunodeficiency (8). Research studies have shown that FoxP3 functions as a tumor suppressor in several types of cancer (9-11).
FoxP3 is crucial for the development of T cells with regulatory properties (Treg) (6). Mutations in FoxP3 are associated with immune dysregulation, polyendocrinopathy, enteropathy, and X-linked syndrome (IPEX) (7), while overexpression in mice causes severe immunodeficiency (8). Research studies have shown that FoxP3 functions as a tumor suppressor in several types of cancer (9-11).
- Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
- Shu, W. et al. (2001) J Biol Chem 276, 27488-97.
- Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8.
- Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65.
- Li, S. et al. (2004) Mol Cell Biol 24, 809-22.
- Ochs, H.D. et al. (2007) Immunol Res 38, 112-21.
- Bennett, C.L. et al. (2001) Nat Genet 27, 20-1.
- Kasprowicz, D.J. et al. (2003) J Immunol 171, 1216-23.
- Zuo, T. et al. (2007) Cell 129, 1275-86.
- Zuo, T. et al. (2007) J Clin Invest 117, 3765-73.
- Wang, L. et al. (2009) Cancer Cell 16, 336-46.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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