FoxM1 Antibody #3948
Inquiry Info. # 3948
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Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
| MW (kDa) | 110 |
| Activity | Differential splicing of exons Va (A1) and VIIa (A2) gives rise to 3 classes of transcripts, which encode 3 different protein isoforms: FoxM1A, containing both alternative exons; FoxM1B, containing none of the alternative exons and FoxM1C, containing only exon Va. This antibody (#3948) will recognize all 3 splice varients. Also see http://atlasgeneticsoncology.org/Genes/FOXM1ID40631ch12p13.htmlFoxM1 protein is stabilized after DNA damage and requires Chk2 kinase and can be stimulated by UV, IR, and etoposide exposure. No change in FoxM1 mRNA levels is observed after DNA damage treatments. Yongjun Tan et. al. 2007. Molecular and Cellular Biology, February, p. 1007-1016, Vol. 27, No. 3 (PMID: 17101782) |
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
FoxM1 Antibody detects endogenous levels of total FoxM1 protein. All three alternative splice variants are predicted to be recognized (FoxM1a, FoxM1b, and FoxM1c).
Species Reactivity:
Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FoxM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Forkhead box M1 (FoxM1) is a forkhead box family transcription factor that regulates a number of genes throughout the cell cycle to help control DNA replication, mitosis, and cell proliferation. FoxM1 expression increases during G1 and S and reaches maximum levels in G2/M (1-3). Nuclear translocation occurs just before entry into G2/M and is associated with FoxM1 phosphorylation (4). Phosphorylation of FoxM1 by MAPK (Ser331, Ser704), Cyclin/Cdk (Ser4, Ser35, Thr600, Thr611, Thr620, Thr627, Ser638), Plk1 (Ser715, Ser724), and Chk2 (Ser376) stabilizes and activates FoxM1 (4-8). Forkhead box M1 is expressed in all embryonic tissues but is restricted to proliferating tissues in adults (9). Research studies show that FoxM1 expression is negatively regulated by p53 (10,11). Upregulation of FoxM1 is associated with many human cancers, including prostate, breast, lung, ovary, colon, pancreas, stomach, bladder, liver, and kidney, and may be associated with p53 mutations in some tumors (11,12). As a result, FoxM1 inhibitors have become a topic of interest for potential cancer therapy (13).
- Wang, I.C. et al. (2005) Mol Cell Biol 25, 10875-94.
- Leung, T.W. et al. (2001) FEBS Lett 507, 59-66.
- Wang, X. et al. (2002) Proc Natl Acad Sci U S A 99, 16881-6.
- Ma, R.Y. et al. (2005) J Cell Sci 118, 795-806.
- Laoukili, J. et al. (2008) Mol Cell Biol 28, 3076-87.
- Fu, Z. et al. (2008) Nat Cell Biol 10, 1076-82.
- Tan, Y. et al. (2007) Mol Cell Biol 27, 1007-16.
- Anders, L. et al. (2011) Cancer Cell 20, 620-34.
- Ye, H. et al. (1997) Mol Cell Biol 17, 1626-41.
- Barsotti, A.M. and Prives, C. (2009) Oncogene 28, 4295-305.
- Pandit, B. et al. (2009) Cell Cycle 8, 3425-7.
- Pilarsky, C. et al. (2004) Neoplasia 6, 744-50.
- Gartel, A.L. (2008) Expert Opin Ther Targets 12, 663-5.
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