R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Flotillin-1 (D2V7J) XP® Rabbit mAb #18634
Filter:
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 49 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #97966.
For a carrier free (BSA and azide free) version of this product see product #97966.
Protocol
Specificity / Sensitivity
Flotillin-1 (D2V7J) XP® Rabbit mAb recognizes endogenous levels of total flotillin-1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile368 of human flotillin-1 protein.
Background
Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry an evolutionarily conserved domain called the prohibitin homology domain (PHB) (1). Flotillin members are ubiquitously expressed and located in noncaveolar microdomains (lipid rafts) on the plasma membrane where they support signal transduction and regulate lipid raft motility and localization (2-5). Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found in compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, and the nucleus (6,7). Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome (4,8,9). Both flotillin-1 and flotillin-2 are commonly used as lipid raft-associated markers.
- Morrow, I.C. and Parton, R.G. (2005) Traffic 6, 725-40.
- Langhorst, M.F. et al. (2005) Cell Mol Life Sci 62, 2228-40.
- Stuermer, C.A. and Plattner, H. (2005) Biochem Soc Symp , 109-18.
- Fernow, I. et al. (2007) Eur J Cell Biol 86, 345-52.
- Neumann-Giesen, C. et al. (2007) J Cell Sci 120, 395-406.
- Liu, J. et al. (2005) J Biol Chem 280, 16125-34.
- Santamaría, A. et al. (2005) Mol Cell Biol 25, 1900-11.
- Solomon, S. et al. (2002) Immunobiology 205, 108-19.
- Neumann-Giesen, C. et al. (2004) Biochem J 378, 509-18.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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