R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
FLASH/CASP8AP2 (D3T8Q) Rabbit mAb #43339
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 223 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
FLASH/CASP8AP2 (D3T8Q) Rabbit mAb recognizes endogenous levels of total FLASH/CASP8AP2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to residues near the amino terminus of human FLASH/CASP8AP2 protein.
Background
FLICE associated huge protein (FLASH), also termed Caspase 8 associated protein 2 (CASP8AP2), is a large multifunctional protein that has been shown to be involved in multiple processes, including apoptosis, cell cycle progression, and transcriptional regulation (1-3). It was originally identified as a component of the FAS-FADD-Caspase 8 complex with a role in the activation of caspase-8 during Fas-mediated apoptosis (1). However, studies suggest additional functions for FLASH/CASP8AP2 in the nucleus within Cajal and PML bodies (3-5). One of the functions identified was its ability to regulate histone gene processing and S-phase progression (3,6). FLASH/CASP8AP2 has also been found to regulate the activity of transcription factors, including the glucocorticoid receptor and c-Myb (2,7). Furthermore, FLASH/CASP8AP2 was found to stabilize the expression of ZEB1, a transcriptional repressor of genes associated with epithelial-to-mesenchymal transition (EMT), a process associated with cancer metastasis (8). Aberrant expression as well as genetic mutations in the FLASH/CASP8AP2 gene have been described in several cancers and it has been suggested to be a prognostic indicator in acute lymphoblastic leukemia (9-14).
- Imai, Y. et al. (1999) Nature 398, 777-85.
- Kino, T. and Chrousos, G.P. (2003) J Biol Chem 278, 3023-9.
- Barcaroli, D. et al. (2006) Proc Natl Acad Sci U S A 103, 14808-12.
- Milovic-Holm, K. et al. (2007) EMBO J 26, 391-401.
- Barcaroli, D. et al. (2006) Proc Natl Acad Sci U S A 103, 14802-7.
- Yang, X.C. et al. (2009) Mol Cell 36, 267-78.
- Alm-Kristiansen, A.H. et al. (2008) Oncogene 27, 4644-56.
- Abshire, C.F. et al. (2016) Oncogenesis 5, e254.
- Jeong, E.G. et al. (2007) APMIS 115, 900-5.
- Park, J. et al. (2002) Cancer Res 62, 1284-8.
- Park, T.S. et al. (2009) Cancer Genet Cytogenet 195, 94-5.
- Flotho, C. et al. (2006) Blood 108, 1050-7.
- Jiao, Y. et al. (2012) Leuk Res 36, 67-71.
- Noronha, E.P. et al. (2019) Front Oncol 9, 316.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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