FKBP5 Antibody #8245
Filter:
- WB
Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 51 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
FKBP5 Antibody recognizes endogenous levels of total FKBP5 protein. This antibody does not cross-react with FKBP4 protein.
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FKBP5 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
FK506 binding protein 51 (FKBP51, also called FKBP5) belongs to the FKBP family of immunophilins (1). FKBP family proteins contain FK domains and TPR (tetratricopeptide repeat) domains. The FK domains are responsible for PPIase (peptidylprolyl isomerase) activity and allow binding to FK506 and rapamycin (2,3). The C terminal TPR domains are involved in protein-protein interactions. The TPR domain of FKBP5 mediates binding to HSP90 complexes (4), as well as glucocorticoid, androgen, and progesterone receptors, which account for its regulatory role in steroid hormone receptor function (5). FKBP5 also binds to IKKα and is involved in NF-κB signaling (6,7). In addition, FKBP5 was identified as a negative regulator of Akt through promotion of Akt - PHLPP interaction and enhanced dephosphorylation of Akt (8).
- Avilés, A. et al. (1995) Clin Oncol (R Coll Radiol) 7, 97-101.
- Fruman, D.A. et al. (1994) FASEB J 8, 391-400.
- Fix, M.L. et al. (2008) Blood Press Monit 13, 199-203.
- Scheufler, C. et al. (2000) Cell 101, 199-210.
- Schülke, J.P. et al. (2010) PLoS One 5, e11717.
- Bouwmeester, T. et al. (2004) Nat Cell Biol 6, 97-105.
- Avellino, R. et al. (2005) Blood 106, 1400-6.
- Pei, H. et al. (2009) Cancer Cell 16, 259-66.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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