R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
FGF19 (D1N3R) Rabbit mAb #83348
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
FGF19 (D1N3R) Rabbit mAb recognizes endogenous levels of total human FGF19 protein. This antibody does not cross-react with the mouse ortholog FGF15.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala148 of human FGF19 protein.
Background
FGF19 is a member of the large and diverse Fibroblast Growth Factor (FGF) family of peptide growth factors. FGF19 functions as a high affinity ligand for the FGF4 receptor, to which it binds in a heparin-dependent manner (1). Under normal physiological conditions, FGF19 is produced in the ileum when absorbed bile acids bind to the farnesoid X receptor (FXR), activating transcription of the FGF19 gene. FGF19 in turn functions in a negative feedback fashion to regulate bile acid synthesis (2). Consequently, disruptions in FGF19 signaling have been linked with clinical defects in bile acid synthesis, which may manifest as primary bile acid diarrhea or cholestasis (3,4). Research studies in oncology have shown that FGF19 can function in an endocrine, paracrine or autocrine fashion to promote tumorigenesis. In human studies, this has been demonstrated for breast cancer (5), gastric cancer (6) prostate cancer (7, 8), and hepatocellular carcinoma (9). Similar tumorigenic effects have been described for the mouse ortholog (Fgf15), notably in genetic models of hepatocellular carcinoma (10, 11)
- Xie, M.H. et al. (1999) Cytokine 11, 729-35.
- Miyata, M. et al. (2012) J Steroid Biochem Mol Biol 132, 41-7.
- Walters, J.R. et al. (2009) Clin Gastroenterol Hepatol 7, 1189-94.
- Schaap, F.G. et al. (2009) Hepatology 49, 1228-35.
- Tiong, K.H. et al. (2016) Oncotarget 7, 57633-50.
- Wang, S. et al. (2016) Oncol Res 23, 197-203.
- Nagamatsu, H. et al. (2015) Prostate 75, 1092-101.
- Feng, S. et al. (2013) Cancer Res 73, 2551-62.
- Sawey, E.T. et al. (2011) Cancer Cell 19, 347-58.
- Desnoyers, L.R. et al. (2008) Oncogene 27, 85-97.
- Uriarte, I. et al. (2015) Int J Cancer 136, 2469-75.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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