Fetuin A (F180) Antibody #5258
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45-50 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Fetuin A (F180) Antibody detects endogenous levels of total fetuin A protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe180 of human fetuin A protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Fetuin A is produced by liver and secreted to plasma, and selectively concentrated in bone matrix. It is synthesized as a precursor and processed to generate 2 chains that are held together by a disulfide bond. It is a systemically acting calcification inhibitor (1). Reduced level of fetuin A in serum is associated with increased cardiovascular mortality in dialysis patients (2,3). Fetuin A can inhibit insulin-induced tyrosine phosphorylation of the insulin receptor tyrosine kinase and insulin receptor substrate-1 (4). Fetuin A deficient mice show high insulin sensitivity and high levels of serum fetuin A are associated with insulin resistance in humans (5,6).
- Schafer, C. et al. (2003) J Clin Invest 112, 357-66.
- Westenfeld, R. et al. (2007) Trends Cardiovasc Med 17, 124-8.
- Westenfeld, R. et al. (2009) J Am Soc Nephrol 20, 1264-74.
- Auberger, P. et al. (1989) Cell 58, 631-40.
- Mathews, S.T. et al. (2002) Diabetes 51, 2450-8.
- Stefan, N. et al. (2006) Diabetes Care 29, 853-7.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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