FEN-1 Antibody #2746
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
FEN-1 Antibody detects endogenous levels of total FEN-1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Lys313 of human FEN-1. Antibodies are purified by peptide affinity chromatography.
Background
Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways (1,2). The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers (3). FEN-1 is also required for non-homologous end joining of double-stranded DNA breaks in long patch base excision repair (4,5). The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions (6,7), post-translational modifications (8,9), and subcellular re-localization in response to cell cycle or DNA damage (10).
- Shen, B. et al. (2005) Bioessays 27, 717-29.
- Liu, Y. et al. (2004) Annu. Rev. Biochem. 73, 589-615.
- Sakurai, S. et al. (2005) EMBO J. 24, 683-93.
- Wu, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 1303-8.
- Gary, R. et al. (1999) J. Biol. Chem. 274, 4354-63.
- Brosh, R.M. et al. (2001) EMBO J. 20, 5791-801.
- Sharma, S. et al. (2004) J. Biol. Chem. 279, 9847-56.
- Henneke, G. et al. (2003) Oncogene 22, 4301-13.
- Hasan, S. et al. (2001) Mol. Cell 7, 1221-31.
- Qiu, J. et al. (2001) J. Biol. Chem. 276, 4901-8.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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