R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
FcRγ (E6Y1A) Rabbit mAb #78401
Filter:
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 12 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| IHC Leica Bond | 1:400 - 1:1600 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Immunofluorescence (Frozen) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #98492.
For a carrier free (BSA and azide free) version of this product see product #98492.
Protocol
Specificity / Sensitivity
FcRγ (E6Y1A) Rabbit mAb recognizes endogenous levels of total FcRγ protein. Non-specific staining was observed in normal human kidney and salivary gland by immunohistochemistry.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FcRγ protein.
Background
FcRγ (Fc receptor gamma-chain, FcεRI gamma, High affinity immunoglobulin epsilon receptor subunit gamma) is a component of the IgE Fc receptor FcεRI, a tetramer of an alpha chain, a beta chain, and two disulfide-linked gamma chains (FcRγ) (1,2). FcεRI is expressed exclusively on basophils and mast cells and is involved in triggering allergic reactions (1-3). FcRγ also forms signaling complexes with other Fc receptors, including FcγRIA and FcγRIIIA. FcRγ shows high homology to the T cell receptor (TCR) ζ chain (4-6) and contains an immunoreceptor tyrosine-based activation motif (ITAM) which elicits a pro-inflammatory response (1-3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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