渲染靶标:SSR
Render Timestamp: 2025-01-08T01:54:09.183Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-10-16 17:30:23.667
Product last modified at: 2024-12-23T13:00:14.939Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

FAP (F1A4G) Rabbit mAb #52818

Filter:
  • WB
  • IP
  • IHC
  • F
Western Blotting Image 1: FAP (F1A4G) Rabbit mAb
Western blot analysis of extracts from various cell lines using FAP (F1A4G) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of FAP protein in SK-MEL-28 cells is consistent with the predicted expression pattern.

To Purchase # 52818T

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 90
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:100
Immunohistochemistry (Paraffin) 1:80 - 1:320
Flow Cytometry (Live) 1:500 - 1:2000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #81192.

Protocol

Specificity / Sensitivity

FAP (F1A4G) Rabbit mAb recognizes endogenous levels of total FAP protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human FAP protein.

Background

Fibroblast activation protein/seprase (FAP) is a cell surface serine integral membrane protease with roles in tissue remodeling during development and wound healing, as well as in cancer and fibrotic disease. FAP has both dipeptidyl peptidase and endopeptidase activities, and cleaves at N-terminal Xaa-Pro sequences. FAP is highly expressed in tumor stroma, and contributes to migration and invasion of tumor cells, as well as that of endothelial cells, into the extracellular matrix. FAP is a useful marker of tumorigenic stroma and of cancer-associated fibroblasts (CAFs) and fibrosis (1,2).
For Research Use Only. Not For Use In Diagnostic Procedures.
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