R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ETV5 (E5G9V) Rabbit mAb #16274
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 68 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ETV5 (E5G9V) Rabbit mAb recognizes endogenous levels of total ETV5 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human ETV5 protein.
Background
ETV5 (ERM) is a member of the PEA3 (polyomavirus enhancer activator 3) subfamily of the E26 transformation-specific (ETS) DNA-binding transcription factors (1,2). PEA3 subfamily members are considered as oncogenic transcriptional factors due to their close association with and contribution to cancer development. The subfamily of ETS proteins consists of three members: ETV1, ETV4, and ETV5. RAS/MAPK and PKA are identified to be the upstream activator of ETV5 by phosphorylation of the target at serine residues (3-5). In addition to cancer promoting effect, ETV5 also regulates many basic cellular functions during normal development, including embryonic stem cell differentiation (6,7), neuronal cell growth, differentiation, and neuron-dendrite network establishment (8,9), reproduction organ development (10,11), as well as cellular fatty acid metabolism and obesity (12,13).
- Qi, T. et al. (2020) Am J Cancer Res 10, 3083-3105.
- Oh, S. et al. (2012) Biochim Biophys Acta 1826, 1-12.
- O'Hagan, R.C. et al. (1996) Oncogene 13, 1323-33.
- Janknecht, R. et al. (1996) Oncogene 13, 1745-54.
- Baert, J.L. et al. (2002) J Biol Chem 277, 1002-12.
- Akagi, T. et al. (2015) J Biol Chem 290, 22460-73.
- Kalkan, T. et al. (2019) Cell Stem Cell 24, 785-801.e7.
- Fontanet, P. et al. (2013) J Neurosci 33, 15940-51.
- Fontanet, P.A. et al. (2018) Cereb Cortex 28, 236-249.
- Eo, J. et al. (2012) Clin Exp Reprod Med 39, 41-5.
- Eo, J. et al. (2011) Mol Hum Reprod 17, 568-76.
- Mao, Z. et al. (2021) Diabetes 70, 214-226.
- Gutierrez-Aguilar, R. et al. (2015) Physiol Behav 150, 38-42.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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