R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ETV4 (E5Q1U) Rabbit mAb #19200
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 54-61 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ETV4 (E5Q1U) Rabbit mAb recognizes endogenous levels of total ETV4 protein. This antibody does not cross-react with ETV5 protein. Based on homology and observed expression, this antibody is not predicted to cross-react with ETV1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human ETV4 protein. The antigen has been further categorized as corresponding to residues surrounding Gly144 of human ETV4 protein.
Background
ETS translocation variant 4 (ETV4, PEA3, E1AF) is a member of the PEA3 (polyomavirus enhancer activator 3) subfamily of the E26 transformation-specific (ETS) DNA-binding transcription factors, which commonly bind a core DNA sequence of 5'-GGA(A/T)-3' (1,2). The PEA3 subfamily of ETV1, ETV4, and ETV5 are considered oncogenic transcription factors due to their overexpression and promotion of tumorigenesis in numerous cancers (2,3). ETV4 can be activated via the RAS-RAF-MEK-ERK pathway (2,4) or the PI3K/Akt pathway (5), and loss-of-function mutations of PEA3-family repressors such as CIC can increase ETV4 expression independent of either pathway (2,6). Though rare, 5' chromosomal fusions to ETV4 are drivers of metastasis in prostate cancer (7) and Ewing sarcoma (8). ETV4 regulates the expression of genes that control several developmental processes, including cell growth, differentiation, proliferation, survival, and cell cycle progression (9-11).
- Oh, S. et al. (2012) Biochim Biophys Acta 1826, 1-12.
- Qi, T. et al. (2020) Am J Cancer Res 10, 3083-3105.
- Jiang, W. et al. (2021) Mol Ther Oncolytics 22, 518-527.
- DeSalvo, J. et al. (2021) J Clin Invest 131, e141908. doi: 10.1172/JCI141908.
- Wang, J. et al. (2022) Oncol Lett 23, 41.
- Hung, Y.P. et al. (2016) Mod Pathol 29, 1324-1334.
- Tomlins, S.A. et al. (2006) Cancer Res 66, 3396-400.
- Boone, M.A. et al. (2021) Mol Cancer Res 19, 1795-1801.
- Xu, L. et al. (2020) Cancer Lett 482, 74-89.
- Yao, D. et al. (2021) Cell Biol Int 45, 2129-2139.
- Tyagi, N. et al. (2018) Mol Cancer Res 16, 187-196.
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