R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
EREG (D4O5I) Rabbit mAb #12048
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 17,19, 30 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
EREG (D4O5I) Rabbit mAb recognizes endogenous levels of proepiregulin and the C-terminal propeptide of the EREG protein. It does not recognize the mature form of EREG.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu155 of human EREG protein.
Background
Epiregulin (EREG) belongs to the epidermal growth factor (EGF) family and is a ligand for the EGF receptor (EGFR) and ErbB4 (1-3). The binding of EREG to homodimers, as well as heterodimers containing EGFR or ErbB4, leads to receptor activation and downstream signaling to promote cell growth and proliferation (4-6). In normal human tissue, moderate levels of EREG are expressed in the placenta and peripheral blood macrophages. Research studies have shown that EREG is expressed at high levels in numerous cancer cell lines, and EREG expression is correlated with primary cancer aggresiveness/metastases (7-11). In addition to its involvement in tumorigenesis, a variant of EREG has also been shown to be associated with tuberculosis susceptibility (12). EREG is synthesized as a ~30 kDa glycosylated membrane bound proepiregulin form (19 kDa when not glycosylated) and through subsequent proteolytic cleavage is processed to a 17 kDa C-terminal propeptide, and the 6kDa mature form of epiregulin (13).
- Olayioye, M.A. et al. (2000) EMBO J 19, 3159-67.
- Shelly, M. et al. (1998) J Biol Chem 273, 10496-505.
- Komurasaki, T. et al. (1997) Oncogene 15, 2841-8.
- Komurasaki, T. et al. (2002) Growth Factors 20, 61-9.
- Shirakata, Y. et al. (2000) J Biol Chem 275, 5748-53.
- Toyoda, H. et al. (1995) J Biol Chem 270, 7495-500.
- Toyoda, H. et al. (1997) Biochem J 326 ( Pt 1), 69-75.
- Zhu, Z. et al. (2000) Biochem Biophys Res Commun 273, 1019-24.
- Kuramochi, H. et al. (2012) BMC Cancer 12, 88.
- Zhang, J. et al. (2008) Cancer Prev Res (Phila) 1, 201-7.
- Sunaga, N. et al. (2012) Oncogene , .
- Thuong, N.T. et al. (2012) Genes Immun 13, 275-81.
- Baba, I. et al. (2000) Cancer Res 60, 6886-9.
限制使用
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