R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ERCC1 (D61F5) Rabbit mAb #5437
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 39 |
| Source/Isotype | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ERCC1 (D61F5) Rabbit mAb recognizes endogenous levels of total ERCC1 isoforms 1, 2 and 3 protein. Experiments utilizing overexpression constructs indicated that this antibody does not cross-react with ERCC1 isoform 4.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ERCC1 protein.
Background
DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals (1). Excision Repair Cross Complementing 1 (ERCC1) forms a complex with ERCC4/XPF, which acts as the 5’ endonuclease required to excise the lesion (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC) (5,6).
- Shuck, S.C. et al. (2008) Cell Res 18, 64-72.
- McDaniel, L.D. and Schultz, R.A. (2008) Adv Exp Med Biol 637, 65-82.
- Niedernhofer, L.J. et al. (2004) Mol Cell Biol 24, 5776-87.
- Ahmad, A. et al. (2008) Mol Cell Biol 28, 5082-92.
- Zheng, Z. et al. (2007) N Engl J Med 356, 800-8.
- Gossage, L. and Madhusudan, S. (2007) Cancer Treat Rev 33, 565-77.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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