ENPP3/CD203c Antibody #75442
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ENPP3/CD203c Antibody recognizes endogenous levels of total ENPP3/CD203c protein. This antibody does not cross-react with either ENPP1 or ENPP2 proteins.
Species Reactivity:
Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ENPP3/CD203c protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3/CD203c/PD-Ιbeta) is a type-II transmembrane glycoprotein that contains a large extracellular domain, an α-helical transmembrane segment, and a short cytoplasmic domain containing the N-terminus. ENPP3 has been shown to be overexpressed in colon carcinoma and is thought to play a role in tumor initiation and tumor cell invasiveness (1-3). Within the hematopoietic cell compartment, ENPP3 is a cell surface marker of basophil and mast cell lineages (4,5). Indeed, ENPP3 is overexpressed on transformed mast cells in patients with systemic mastocytosis (6). Recently, ENPP3 has been identified as being highly overexpressed in renal cell carcinoma (RCC) and may have potential as a novel therapeutic target for this disease (7).
- Yano, Y. et al. (2003) Int J Mol Med 12, 763-6.
- Meerson, N.R. et al. (2000) J Cell Sci 113 Pt 23, 4193-202.
- Deissler, H. et al. (1999) FASEB J 13, 657-66.
- Bühring, H.J. et al. (1999) Blood 94, 2343-56.
- Bühring, H.J. et al. (2001) Blood 97, 3303-5.
- Hauswirth, A.W. et al. Int J Immunopathol Pharmacol 21, 797-806.
- Doñate, F. et al. (2016) Clin Cancer Res 22, 1989-99.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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